SIDT2 Knockout cell line (HEK293)

SIDT2 Knockout cell line (HEK293) Order

Catalog#

YKO-XN71142

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

SIDT2 Knockout cell line (HEK293)

Morphology

Epithelial-like, adherent

Passage ratio

1:3~1:6

Species

Human

Gene Name

SIDT2

Gene ID

51092

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90%DMEM+10% FBS

Target region

Knockout region: Exon 1 (based on transcript SIDT2-202)
Validation: PCR+Sanger Sequencing

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the SIDT2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HEK293 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, SIDT2 gene knockout SIDT2 Knockout cell line (HEK293) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

SIDT2 Knockout cell line (HEK293)

Catalog#

YKO-XN71142

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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SIDT2 Knockout cell line (HEK293)

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